QUANTITATIVE DETERMINATION OF BENZOIC, HIPPURIC, AND PHENACETURIC ACIDS IN URINE

The estimation of hippuric and phenaceturic acids in urine.

QUANTITATIVE DETERMINATION OF BENZOIC, HIP- PURIC, AND PHENACETURIC ACIDS IN UR1NE.l

Since 1824 when Wijhler first called attention to the fact that the formation of hippuric acid represented a synthetic reaction by the animal body, this substance has occupied an interesting place in biological chemistry. In recent years it has received special attention from the fact that the study of its production may throw light upon certain phases of nitrogen metabolism. However, owing to ...

Quantitative determination of hippuric and m-methylhippuric acids in urine by high-speed liquid chromatography.

High-speed liquid chromatography employing an ultraviolet photometric detector has been applied to the simultaneous determination of hippuric and m-methylhippuric acids in urine. Reversed-phase partition chromatography is carried out on a muBondapak C18 column with methanol-water as the eluent system. This method obviates the necessity for isolation or reaction of these acids before assay. The ...

Colorimetric determination of hippuric acid in urine and liver homogenate.

To a dried extract Containing hippuric acid (HA), 1.0 mL of acetic anhydride and 2.0 mL of 0.5% p-dlmethyiamlnobenzaldehyde (DAB) solution In pyridine were added, and the solution was kept at 40 O C for 1 h after thorough mlxing. The absorbance was then determlned at 458 nm agalnst a blank containing acetic anhydride, DAB, and pyridine. This method was in good agreement with Beer’s law within 1...

A modified method for the determination of hippuric acid and free benzoic acid in the urine of cattle.